When Biostimulants Tip the Balance: Lessons from Callus Formation in Sensitive Crops

Biostimulants are valuable tools in horticulture. They can improve rooting, enhance resilience, and support stress recovery. But just like with fertilizers or crop protection products, the right dose and the right combination are crucial.

In a recent case, callus tissue developed in young pot roses. Callus is disorganized, undifferentiated plant tissue — essentially the plant “switching on” regeneration mode. While this may sound beneficial, in practice it can lead to poor root development, growth abnormalities, and weaker plants.

After ruling out pathogens such as Agrobacterium tumefaciens, the focus turned to cultural practices and inputs. The analysis showed:

  • Electrical Conductivity (EC) was relatively high for rooting cuttings, limiting proper root initiation.
  • Recirculated irrigation water inadvertently carried multiple rounds of biostimulants to young plants.
  • Hormone-rich inputs such as certain seaweed extracts (rich in cytokinins) posed a moderate-to-high risk of callus formation, especially when combined with rooting powders or other stimulants.

Other biostimulants like vermicompost or amino acid mixes had low risk, but the synergy of multiple stimulants together created “too much of a good thing.”

  • Seaweed extracts are powerful, but overdosing or doubling up with similar products can unbalance auxin–cytokinin ratios, triggering callus instead of healthy roots.
  • Combining stimulants without clear strategy can cause antagonistic or unpredictable effects.
  • Safer, complementary combinations include worm compost + amino acids (for soil and nutrition) or chitosan + amino acids (for stress management).
  • In propagation, choose either a rooting powder or a hormonal biostimulant — rarely both.

Case Study: When Growing Conditions Tip the Balance in Xanthomonas Infections

A few years ago, strawberry plants of a well-known cultivar were propagated under controlled conditions. Inspections during the season found nothing unusual — clean mother plants, a new tray field, no water recirculation, and routine checks all gave the green light.

Yet once plants were distributed, a puzzling pattern emerged. Some growers reported heavy outbreaks of Xanthomonas fragariae, while others with plants from the very same source saw little to no issue.

Why such a difference? The answer lies not only in the presence of the bacterium, but in the growing environment that favors its development.

  • The bacterium enters leaves through stomata and thrives in high humidity with free moisture on foliage.
  • Temperatures of 18–24 °C accelerate its growth.
  • Young, tender leaves are especially vulnerable.
  • High nitrogen fertilization makes plants lush, but also more susceptible.
  • Water splash and crop handling can move the bacterium from plant to plant.

In some fields, these conditions lined up perfectly — turning a hidden, symptomless infection into a rapid epidemic. In others, the environment was less favorable, and the disease failed to establish.

  • Even when plant material appears “clean,” opportunistic pathogens can strike if conditions align.
  • Strict sanitation, careful nitrogen management, and minimizing leaf wetness are essential preventive steps.
  • Early detection through scouting and diagnostic testing helps prevent widespread spread.

The key insight: Xanthomonas infections are as much about the crop’s environment as about the pathogen itself. A clean start is important, but staying clean requires vigilance against the growing conditions that give the bacterium its opportunity.

How Risk Assessment Helped Uncover the Source of a Plant Health Crisis

In a recent project, large-scale plant losses in asparagus production raised urgent questions. Symptoms included shortened and fibrous root crowns, cracked and discolored roots, unpleasant odors, and disrupted stem growth. Lab analysis revealed heavy infections with Fusarium oxysporum and Cylindrocarpon didymum. Although Phytophthora was tested, it was not detected. The strong odor suggested a secondary Erwinia infection.

A structured risk assessment was key to moving beyond symptoms and lab results. During the sessions, we traced the most likely origin of the widespread issue back to a practice a few years earlier: shredding crop residues on the windward side of the growing area. This led to contaminated dust entering the basins and irrigation system. While disinfection systems were in place, no method is 100% effective, especially when particle loads are high and water turbidity reduces efficacy. This provided a pathway for infection across the entire facility.

The assessment also highlighted contributing factors:

  • Prohibition of certain chemical seed treatments, leaving only water-dipping as a preventive step.
  • Movement of plants between plots, increasing cross-contamination risk.
  • An existing background level of infection, amplifying new outbreaks.

With this understanding, we designed a multi-step plan of action:

  • Knowledge transfer on pathogen lifecycles and symptom recognition.
  • Trials combining nutrition strategies with biological controls (“measure to manage”).
  • Revision of hygiene protocols, co-created with cultivation staff for ownership.
  • Optimized plant flow to protect young seedlings.
  • Field sanitation and soil reset trials.
  • Treatment strategies for new plants entering production.
  • Ongoing training in pathogen identification, supported by lab analysis.

Next to tackling the plant health issues, fertilization practices were also adjusted to better support root health and resilience. The results spoke for themselves: already in the first year after implementation, 80% fewer issues were recorded.

The outcome of this process was not only the identification of the likely cause but also a clear pathway to reduce risks and prevent recurrence. By combining scientific analysis with practical on-site risk assessment, it became possible to transform a widespread challenge into a structured recovery plan.

Part 1 Plants Don’t Whisper – They Broadcast

We tend to think of plants as passive. In truth, they’re broadcasting signals all the time. We just have to tune in.

How sugar concentration reflects photosynthesis and plant energy.

Role of sugars in plants

Sugars in plants are formed through photosynthesis, a process where plants use light energy, water and carbon dioxide to create glucose and oxygen. Here is how it happens:

Formation of sugars in leaves: the process occurs in the chloroplasts of plant cells, primarily in leaves. Light is absorbed by chlorophyl, the green pigment, and converst carbon dioxide and water into glucose and oxygen. During daytime, the about of sugars formed is highest between 2 pm and 4 pm.
After glucose is produced in the leaves, it is often converted into sucrose (a disachharide sugar( for transport through the plant. Sucrose ios more stable and can be moved mopre efficiently over long distances.
Sucrose is tranpotered through the phloem vessels to different parts of the plant, including roots, stems, flowers, and growing parts. This movement is driven by pressure differences between source (leaves) and sink (growing tissues) areas.
Some of the sugars may be stored in roots or other storage tissues as starch, which can be converted back to sugar when needed.

Sugars are used for energy in respiration, a process that breaks down glucose into energy (ATP) that powers cellular activities, including growth.

Sugars also serve as building blocks for other compounds like cellulose, which makes up the cell wall, or amino acids, which are used to build proteins. This is crucial for the growth of new tissues, including leaves, stems, and flowers.

Brix is a scale used to measure the concentration of dissolved sugars in a liquid, particularly in plant sap or fruit juice. It represents the percentage of sucrose by weight in the solution.

Plants emit volatile organic compounds to warn neighbors or call in help.

Plants have developed sophisticated communication mechanisms to respond to environmental stimuli, interact with each other, and even defend against threats. Two major ways plants send messages are through volatile organic compounds (VOCs) released into the air and root exudates released into the soil.

How VOC’s work….

When a plant is under stress, such as from herbivore attack, mechanical damage, or environmental stress, it may release VOCs into the atmosphere. These compounds can be emitted by leaves, stems, or flowers. There are different types of VOC’s
Herbivore-Induced Volatiles: These are released when a plant is grazed or damaged by herbivores. They act as distress signals to nearby plants, which may then activate their own defense mechanisms (e.g., producing chemicals to deter herbivores or attract natural predators of herbivores).
Plant-Plant Communication: Plants can also release VOCs that alert nearby plants about environmental changes, like drought or temperature stress, allowing neighboring plants to initiate protective responses.
Attracting Pollinators: Some plants release pleasant-smelling volatiles to attract pollinators like bees, butterflies, or birds.

VOCs act as airborne signals that are detected by receptors in other plants or organisms (like insects). These signals can trigger defence responses and attract beneficial organisms, providing an indirect defence mechanism.

Plants use soil chemistry to communicate with microbes and other plants.

Root exudates are compounds released by plant roots into the surrounding soil. These exudates are a form of communication with the soil microbiome, other plants, and even herbivores.

How root exudates work….

Roots excrete a variety of substances, including sugars, amino acids, organic acids, phenolic compounds, and secondary metabolites. This process can occur under normal conditions or in response to specific stimuli (like pathogen attack or nutrient deficiency).
Nutrient-Rich Compounds: Plants excrete sugars, amino acids, and organic acids to nourish beneficial microbes like mycorrhizal fungi, which help with nutrient uptake (especially phosphorus).
Allelopathic Compounds: Some plants release chemicals into the soil that inhibit the growth of nearby plants, reducing competition for resources (this is known as allelopathy).
Signal Molecules: In response to pathogens or other stressors, plants may release signaling molecules (like jasmonic acid or salicylic acid), which influence the microbial community around their roots and help in activating defense pathways.

Plants don’t only use one form of communication – they often use both. An example: when plants release VOC’s in response to herbivory, these signals can also affect microbial populations in the soil around the roots, increasing the plant’s resistance to pathogens or pests.

Plants send action alerts from leaf to leaf, preparing tissues for defence.

While volatiles and root exudates are primarily chemical signals, they can be translated into electrical signals through the plant’s biochemical processes. The key translation happens through ion fluxes across cell membranes, which alters the plant’s electropotential and generates electrical currents that propagate through the plant. These electrical signals are crucial for the plant’s response to environmental changes, herbivory, and pathogen attacks, allowing it to activate defense mechanisms or adjust growth processes accordingly.


Start tracking brix levels and noting changes in plant appearance before visible damage. Even small shifts can mean stress.

Wilting Strawberry Plants? Don’t Just Blame Neopestalotiopsis

Positive PCR but no active growing Neopestalotiopsis

When strawberry plants start to wilt, it’s easy to point the finger at Neopestalotiopsis. Especially when PCR results come back positive. But let’s take a moment to understand what that actually means. PCR confirms the presence of DNA, not necessarily a living, active fungus. So yes, Neopestalotiopsis might be there – or it was there – but it’s not the whole story.

Plating Neopestalotiopsis

When we plate these plants, we often see a much more complex picture. Multiple pathogens show up: Fusarium spp., Colletotrichum, Cylindrocarpon, Phytophthora, and Neopestalotiopsis. In other words, this is not a single-pathogen issue. We’re dealing with a disease complex – and more importantly, a root rot – above ground disease complex.

Focusing only on leaf infections caused by Neopestalotiopsis could lead you to overlook the more serious threat lurking below ground. Root rot quietly wipes out your plants, and by the time it becomes visible, it’s often too late.

Going heavy on fungicides might seem like a quick fix, but be careful, this can backfire. Overapplication leads to extra plant stress, and stressed plants are more vulnerable to root pathogens. Plants can’t fight on two fronts at once. If all their energy goes into managing chemical stress and foliar infections, the roots become an easy target.

I have been analysing plants in the Netherlands as well as Canada. They tell the same story. We have a disease complex, not just Neo-P.

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